What are the advantages of using DBCO-based "Click Chemistry" for conjugation?

For more: DBCO-SS-NHS

Copper-Free: Unlike traditional click chemistry, SPAAC does not require toxic copper catalysts, making it safer for biological molecules and live cells.

High Efficiency: The reaction is fast, highly specific, and can be performed under physiological conditions.

Site-Specificity: When used with azide-modified antibodies, it allows for more controlled and uniform conjugation.